Plant Transcription Factor Database
Previous version: v3.0
Nicotiana tabacum
ZF-HD Family
Species TF ID Description
XP_016432582.1ZF-HD family protein
XP_016433661.1ZF-HD family protein
XP_016437739.1ZF-HD family protein
XP_016437760.1ZF-HD family protein
XP_016437905.1ZF-HD family protein
XP_016439200.1ZF-HD family protein
XP_016440003.1ZF-HD family protein
XP_016445401.1ZF-HD family protein
XP_016451406.1ZF-HD family protein
XP_016451603.1ZF-HD family protein
XP_016452911.1ZF-HD family protein
XP_016453067.1ZF-HD family protein
XP_016453079.1ZF-HD family protein
XP_016453087.1ZF-HD family protein
XP_016455169.1ZF-HD family protein
XP_016456660.1ZF-HD family protein
XP_016462879.1ZF-HD family protein
XP_016462880.1ZF-HD family protein
XP_016467621.1ZF-HD family protein
XP_016471282.1ZF-HD family protein
XP_016472938.1ZF-HD family protein
XP_016475302.1ZF-HD family protein
XP_016478339.1ZF-HD family protein
XP_016480030.1ZF-HD family protein
XP_016480031.1ZF-HD family protein
XP_016482443.1ZF-HD family protein
XP_016482481.1ZF-HD family protein
XP_016482483.1ZF-HD family protein
XP_016483695.1ZF-HD family protein
XP_016483696.1ZF-HD family protein
XP_016483697.1ZF-HD family protein
XP_016483698.1ZF-HD family protein
XP_016483701.1ZF-HD family protein
XP_016490581.1ZF-HD family protein
XP_016490986.1ZF-HD family protein
XP_016491455.1ZF-HD family protein
XP_016491456.1ZF-HD family protein
XP_016491457.1ZF-HD family protein
XP_016500216.1ZF-HD family protein
XP_016500955.1ZF-HD family protein
XP_016501840.1ZF-HD family protein
XP_016501841.1ZF-HD family protein
XP_016501975.1ZF-HD family protein
XP_016507066.1ZF-HD family protein
XP_016510094.1ZF-HD family protein
XP_016511425.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511